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Cell Culture

By Greiner Bio-One

Greiner Bio-One GmbH develops and distributes products for the cultivation, storage and separation of cells and for specific applications in medical, pharmaceutical and biotechnological research.

The company's product line CELLSTAR® offers a wide spectrum of cell culture flasks, dishes and plates. Their functional design, particular material properties and special coatings enable these vessels to be used not only in standard cell culture but also in cancer research, viral diagnostics, genetic engineering and vaccine production.

In response to the increasing automation of cell culture procedures, Greiner Bio-One developed the AutoFlask™, a cell culture flask for automated systems. With the ThinCert™Plate and appropriate cell culture inserts, Greiner Bio-One has also created an innovative platform for compatibility tests using skin models in the chemical and cosmetic industries.

The Cell Culture product sector was recently expanded with the innovative Advanced TC™ polymer modification. These cell culture products have a special surface that enables more effective cultivation of fastidious cells and facilitates complex testing systems.

For high-resolution microscopy and live cell analysis, Greiner Bio-One offers the new CELLview™ dish, a polystyrene cell culture dish with an embedded autofluorescence-free glass bottom. The unique subdivided version of the dish is ideally suited for multiplex analyses, for example, and is also available with the Advanced TC™ surface.

CELLSTAR®

Cell culture vessels with physically modified surfaces for adherent or suspension cell cultures.

CELLMASTER™

Roller bottles made of polystyrene (with physical surface treatment) or polyethylene terephthalate.

CELLCOAT®

Protein-coated culture vessels for adherent cell culture. Our CELLSTAR® product family is coated with the following proteins: Collagen Type I, Poly-D-Lysine, Poly-L-Lysine, Fibronectin and Laminin.

ThinCert™ Cell Culture Inserts

Membrane supports for multiwell plates, consisting of polystyrene housings and sealed PET capillary pore membranes. With the ThinCert™Plate, Greiner Bio-One offers an innovative solution for the air-lift culture with ThinCert™ cell culture inserts. Its deep wells allow a larger volume of medium to be available to the air-lift culture.

CELLview™ - Cell culture dish with glass bottom

CELLview™ combines the convenience of a standard size 35mm disposable plastic cell culture dish with the optical quality of glass, providing superior high-resolution microscopic images of in-vitro cultivated cultures.

The innovative design of the cell culture dish provides a single-plane, flat bottom with a consistent working distance and maximal planarity. Moreover, the dish bottom configuration facilitates optimal thermal conductivity and avoids thermal variations in heated platforms used for live cell imaging. The subdivided version of the CELLview™ enables simultaneous multiplex analyses of different cell lines, various stimulations or diverse transfections. Quartering the cell culture dish provides four individual compartments with a growth area of approximately 1.9cm2, allowing minimisation of cells and reagents required per individual assay.

Applications:

  • Phase contrast microscopy
  • Fluorescence microscopy
  • Confocal microscopy
  • Live cell imaging
  • Differential interference contrast microscopy
  • Polarised light microscopy
  • Fluorescence-in-situhybridisation (FISH)

Advanced TC™ Cell Culture Vessels

For the propagation of fastidious cells like primary or sensitive cells as well as cells cultivated under restricted growth conditions (serum-free or serum-reduced) Greiner Bio-One offers the novel Advanced TC™ polymer modification. Based on an innovative technique, the cell culture surface is modified to promote cellular features and functions. Enhanced cell attachment and higher proliferation rates improve and accelerate cell expansion. Furthermore, the Advanced TC™ surface facilitates consistent and homogenous cell attachment increasing the overall cell yield and reducing cell loss, for example during automated washing steps.

Due to the production process, the chemical modification of the polymer assures consistant and reproducible product quality. Transport and storage can be carried out at room temperature.

Advantages:

  • Improved cell adherence
  • Consistent cell attachment
  • Homogenous cell growth
  • In-vivo like morphology
  • Increased cell yield
  • Optimal cultivation conditions for sensitive cells
  • Permits usage of serum-reduced or serum-free media
  • Reduced cell loss due to (automated) washing steps
  • Improved assay consistency
  • Storage at room temperature
  • Two-year shelf life

Applications:

  • Cultivation of fastidious and sensitive cells
  • Usage of serum-reduced or serum-free media
  • Differentiation of semi-adherent cells
  • Transfection
  • Transduction
  • Automation/High-throughput analysis