The Electrospinning Company develops and manufactures electrospun polymer scaffolds which are incorporated into standard tissue culture plates. These provide an ideal environment for supporting the growth of cells in 3D.
Cells grown in a 3D microenvironment look and behave more like cells in human tissues than those cultured in 2D. They are in contact with other cells and produce their own extra-cellular matrix, which influences tissue-specific gene expression, cell growth and the uptake and metabolism of drugs. Our Mimetix 12-well plate with removable scaffold inserts and Mimetix 6-well plate with hanging inserts are ideal tools for small-scale studies involving for example cell viability assays, fluorescence microscopy of cells in the scaffold, or gene expression analysis.
3D cell-based analysis can be an effective way of providing a more realistic date on the usefulness of drug candidates against tumour cells or their toxicity. In turn, such methods can potentially reduce the risk of failure in expensive clinical trials. Our Mimetix 96-well plate is easy-to-use with minimal adaptation required to shift from 2D to 3D and compatible with standard automated handling and imaging equipment. We have obtained promising data using primary and upcyte® hepatocytes (see case studies for more information).
We have extensive scaffold design and manufacturing capabilities to develop bespoke scaffolds from a variety of polymer and in custom formats, such as aligned fibres (supporting the differentiation of cells which require a particular orientation, such as peripheral nerves and tendons) or spinning 3D shapes. We are currently putting in place a quality management system to be able to supply into the medical device market. Please contact us to discuss the development of electrospun scaffolds for your specific application/personal needs.
The Electrospinning Company
Rutherford Appleton Laboratory
Phone: +44 (0)845 3888856