VERTIS specialises in the preparation of high-quality cDNA from all RNA species, as well as a whole range of variation of organisms, tissues and environmental samples.
VERTIS has developed techniques that enable reliable production of cDNA.
Application |
cDNA synthesis protocol |
| Whole transcriptome analysis with complete coverage of the transcriptomes | Random-primed cDNA |
| Digital gene expression (DEG) – measurement of the expression level of all genes | 3′-fragment cDNA |
| Cap analysis gene expression (CAGE) – for genome-wide identification of transcription start sites (TSSs) | 5′-fragment cDNA |
| Small RNA analysis (e.g. miR) | Small RNA cDNA |
| Parallel analysis of RNA Ends (PARE) – for high-throughput identification of microRNA (miRNA) targets | PARE cDNA (according to German et al. 2009, NATURE PROTOCOLS, VOL.4 NO.3) |
| Preparation of cDNA from low amounts of starting material, down to a few nanograms of total RNA | Micro-quantity cDNA |
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