VERTIS specialises in the preparation of high-quality cDNA from all RNA species, as well as a whole range of variation of organisms, tissues and environmental samples.
VERTIS has developed techniques that enable reliable production of cDNA.
cDNA synthesis protocol
|Whole transcriptome analysis with complete coverage of the transcriptomes||Random-primed cDNA|
|Digital gene expression (DEG) – measurement of the expression level of all genes||3′-fragment cDNA|
|Cap analysis gene expression (CAGE) – for genome-wide identification of transcription start sites (TSSs)||5′-fragment cDNA|
|Small RNA analysis (e.g. miR)||Small RNA cDNA|
|Parallel analysis of RNA Ends (PARE) – for high-throughput identification of microRNA (miRNA) targets||PARE cDNA (according to German et al. 2009, NATURE PROTOCOLS, VOL.4 NO.3)|
|Preparation of cDNA from low amounts of starting material, down to a few nanograms of total RNA||Micro-quantity cDNA|