VERTIS are experts in the isolation of high-quality RNA from valuable and challenging starting materials.
We offer different strategies for the removal of ribosomal RNA (rRNA) molecules from total RNA preparations or for the enrichment of full-length mRNA or of small RNA species.
RNA species |
Enrichment strategy |
Poly(A)+ | Oligo(dT) chromatography |
Full-length mRNA | CAP selection (enzymatic or antibody) |
Small non-coding RNA (sncRNA) | Selective elution from silica gel (< 200nt) |
MicroRNA | Size fractionation (15 -30nt) with an automated fractionation system |
rRNA depleted total RNA | Enzymatic degradation of rRNA or removal or rRNA molecules with subtractive hybridization |
RNA species |
Enrichment strategy |
5’PPP primary transcripts | Enzymatic selection of 5’PPP ends |
Small non-coding RNA (sncRNA) | Selective elution from silica gel (< 200nt) |
rRNA depleted total RNA | Enzymatic degradation of rRNA or removal or rRNA molecules with subtractive hybridisation |
RNA-Analysis with a Shimadzu MultiNA microchip electrophoresis system
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