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Glycotope

GlycoEngineering and Immunotherapeutics

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Determination of Host Cell Contaminants

Glycotope

Host cell contaminants, such as host cell protein or host cell DNA, need to be detected using highly-sensitive methods. Glycotope uses commercially available or custom-made bioassays for the detection of host cell proteins. The level host cell DNA can be determined using threshold or RT-PCR techniques.

Host cell-derived proteins are detected either by generic assays or by special assays developed for the client-specific fermentation and purification scheme. The client-specific assays can be developed on standard ELISA format or for the more sensitive threshold system.

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