The objective of this study was to test if the Mimetix® electrospun scaffold is a suitable tool for the culture of human epithelial breast cancer cells in 3D, which can be used in drug discovery programmes for in vitro toxicity and efficacy assays. Cell viability and growth in 12-well plates and 96-well plates was shown to be highly consistent (cv < 10%) and cells were shown to be more resistant to drug-induced apoptosis following 10 days of proliferation in the Mimetix scaffolds than on 2D tissue culture plastic, indicating that they had assumed a 3D phenotype.