For the outstanding efficiency and scalability, high-performance liquid chromatography (HPLC) has been widely used for purification of high-quality APIs and intermediates in the pharmaceutical industry.

The application has been broadened to the purification of natural extracts, which are often very complex mixtures. This application note presents the preparative HPLC purification of cannabinoids.

The process was developed using a bench-top HPLC instrument and then directly scaled-up to Hipersep^® Pilot with Prochrom^® LC150 (150mm i.d.) for manufacturing.