Tailor Made Clonal Cell Lines

Custom cell lines created and modified with an expression vector of your choice with optimal transgene expression, guaranteed stability, reliable cell banking and ready-to-use in just 4-6 weeks.

Service Comprises

• Transfection/transduction of Your Cell Line with an expression vector of your choice
• Generation of stable cell pools by antibiotic selection.
• Establishment of single cell clones using the Genetix Clone Select Imager
• Small scale expansion of individual clones
• Identification of "High Performers" via quantification of target gene or GOI expression by qRT-PCR and/or Western Blot relative to control cells
• Large scale expansion and cell banking of selected clones
• Final QC: Transgene expression, growth characteristics, long-term cultivation (20 passages) optional: marker gene expression profiling, genotyping, genomic mapping of transgene integration site

Benefits

• Clonal cell lines with optimal transgene expression
• Guaranteed stability of cell clones
• Cell banking of individual cell clones for high inter assay stability
• Frozen cells ready-to-use

Available service

• Clonal Gene Knockdown cell lines
• Clonal Gene Overexpression cell lines
• Clonal Reporter Gene cell lines (e.g. luciferase, fluorescent reporter)
• Clonal FRT cell lines for isogenic transgene expression
• Clonal cell lines for TET ON/TET-OFF inducible gene expression