BD™ Cytometric Bead Array (CBA) system allows users to quantify multiple proteins simultaneously, within the range of 0.274–200pg/mL.
Users can flexibly create their own robust-performing multiplexed assay, or use preconfigured kits, and readout is not restricted to a particular platform. The portfolio includes assays for quantitation of a variety cytokines, chemokines, growth factors, immunoglobulins, and phosphorylated cell signalling proteins.
The BD CBA system uses antibody-coated beads to efficiently capture analytes, and the broad dynamic range of fluorescence detection offered by flow cytometry to acquire data. Because each bead in the array has a unique fluorescence intensity, beads can be mixed and run simultaneously in a single tube (multiplexed).
Multiplexing is especially useful because it maximises the number of proteins that can be analysed in a small amount of sample. Other methods such as ELISA require a similar amount of sample, but can analyse only one protein from the same volume. Because such a small amount of sample is needed, multiplexing reduces hands-on time as well as time to results.
All assays have been characterised for performance in single-plex and multiplex scenarios to ensure consistent data. The antibody pair in each assay is evaluated for dynamic range, sensitivity, and parallel titration to native biological samples
BD™ Cytometric Bead Array (CBA) reagents work with any flow cytometer equipped with 488nm and 633nm lasers. The FCAP Array™ software (v3.0) enables complete analysis of data, enabling users to obtain quantitative results just minutes after performing an experiment.
BD CBA solutions are offered in the following product configurations:
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