TSKgel UP-SW3000, a new 2 micron UHPLC column with 25 nm pore size, is the latest addition to the renowned TSKgel SW series of silica based gel filtration columns. For decades, TSKgel G3000SWXL columns have been the gold standard for QC of therapeutic antibodies in Biopharma. Over 40 years of experience in developing and producing SEC stationary phases are built into the new series of UHPLC columns for the analysis of proteins in the range of 10 to 500 kDa.
TSKgel UP-SW3000 features the same pore size as the well-established TSKgel G3000SWXL. Hence, methods developed on conventional gel filtration columns can easily be transferred to UHPLC technology. The 15 cm long column is used to shorten analysis time while maintaining resolution. The 30 cm column delivers dramatically increased resolution between fragments, monomers, and aggregates. To achieve maximum resolution and fully exploit the potential of the columns the use of a UHPLC system is highly recommended. A so-called DC (direct connect) guard column can be attached directly to the analytical column to minimize extra column band broadening.
TSKgel SW columns stand out from other silica-based high performance size exclusion columns by virtue of their large pore volumes. They are based on highly porous, ultra-pure silica particles, the surface of which has been shielded from interacting with proteins by applying a proprietary surface chemistry. This ensures highest recoveries and accurate quantitation. The validated manufacturing and packing process delivers a reliable batch-to-batch reproducibility.
Discover more on the Tosoh Bioscience dedicated site.