Tosoh Bioscience, a provider of chromatographic solutions for the isolation and analysis of biomolecules, introduced a new Protein A affinity column. TSKgel Protein A-5PW is an affinity HPLC column specifically designed for fast and accurate determination of antibody concentrations during cell line selection or upstream bioprocess optimization and control. Typical runtime is below two minutes.
In many stages of monoclonal antibody (mAb) development, harvest cell culture samples must be screened for their IgG titer. Antibody titer determination by Protein A affinity HPLC is much more robust, reliable and reproducible than enzyme-linked immunosorbent assays (ELISAs). More than 2000 injections per column are easily achieved.
The stationary phase uses the same ligand that is successfully applied in TOYOPEARL Protein A affinity media for antibody purification. The column provides a wide dynamic range from 0.05 to 200 µg IgG for quantitation. This makes it suitable to determine low IgG concentrations during early development as well as high concentrations during process control. It can also be used for small scale mAb purifications for further characterization.