"As researchers seek to understand the genetic drivers and mechanisms of tumor development, progression and therapeutic resistance, there is a growing need for a highly-specific approach to enrich for a wide range of gene targets prior to next generation DNA sequencing. Furthermore, technologies need to work well with an increasing range of sample types, including FFPE tissue and liquid biopsies that assay cell-free DNA in blood and other sample types," said Andrew Barry, Target Enrichment Product Marketing Manager at NEB.
NEBNext Direct is a natural extension of the NEBNext® line of sample preparation kits for next generation sequencing that have consistently pushed the boundaries in producing high quality data from diminishing amounts of DNA and RNA. The NEBNext Direct technology leverages NEB’s deep expertise in enzymology to maximize yields and minimize errors that can be introduced during sample preparation.
Emerging from a "ground-up" redesign of targeted sequencing methodologies, the technology draws from a list of critical features, including the ability to enrich for both strands of DNA targets, unique molecular identifiers (UMIs), a fast, single-day, automation-friendly workflow, and a short, single-probe design for flexible targeting and compatibility with degraded samples. The technology is also very scalable, enabling scientists to move directly from a single gene panel to panels with several hundred genes using the same protocol. Individually-synthesized probes allow for rebalancing to optimize coverage uniformity, detect rare alleles, and maximize sequencing efficiency.
New England Biolabs has already engaged with a number of early access users of the technology. According to Francis Rousseau, Ph.D., Director of Genomics for IntegraGen SA, "The kit and its technology are easy to use and easy to automate, allowing us to get up and running quickly. The protocol itself is fast and efficient to obtain deep coverage of targets, giving homogeneous results for FFPE and frozen tumors."
The NEBNext Direct Cancer HotSpot Panel is the initial output of an ongoing partnership with Directed Genomics to develop novel products for next-generation sequencing applications. Directed Genomics will be presenting data in two research posters at the AACR meeting:
– Application of target enrichment combined with unique molecular identifiers to determine allelic frequencies of human cancers (poster #1387);
– RNA DIRECT: A novel strategy for targeted RNA enrichment of transcripts associated with solid tumors (poster #1398).
For more details about the NEBNext Direct Technology, visit NEBNextDirect.com.
The NEBNext Direct Cancer HotSpot Panel is for research use only.
About New England Biolabs:
Established in the mid 1970’s, New England Biolabs, Inc. (NEB) is the industry leader in the discovery and production of enzymes for molecular biology applications and now offers the largest selection of recombinant and native enzymes for genomic research. NEB continues to expand its product offerings into areas related to PCR, gene expression, sample preparation for next generation sequencing, synthetic biology, glycobiology, epigenetics and RNA analysis. Additionally, NEB is focused on strengthening alliances that enable new technologies to reach key market sectors, including molecular diagnostics development. New England Biolabs is a privately held company, headquartered in Ipswich, MA, and has extensive worldwide distribution through a network of exclusive distributors, agents and seven subsidiaries located in Canada, China, France, Germany, Japan, Singapore and the UK. For more information about New England Biolabs visit www.neb.com.
About Directed Genomics:
Directed Genomics is focused on the development of novel DNA and RNA preparation solutions for next-generation sequencing to enhance the efficiency and accuracy of genomic interrogation in both research and clinical environments.
NEB®, NEW ENGLAND BIOLABS®, and NEBNext® are registered trademarks of New England Biolabs, Inc. NEBNext Direct™ is a trademark of New England Biolabs, Inc.
Directed Genomics™ is a trademark of Directed Genomics.
Deana D. Martin, Ph.D.
Marketing Communications Manager
New England Biolabs
240 County Road Ipswich, MA 01938