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Dye-free quantification of Oragene®/saliva DNA extracts using the Trinean DropSense96® Droplet Reader and cDrop™ Spectral Analysis Software

Published 13 April 2016 | By Trinean

Saliva samples collected with Oragene® self-collection kits from DNA Genotek offer a non-invasive and proven method for accessing high quality and quantity DNA for genomic testing and analysis. Proper quantification of the extracted DNA is a critical step for most downstream applications. Trinean has developed a specific quantification protocol for DNA from Oragene/saliva samples using the DropSense96® droplet reader and the cDrop™ spectral decomposition software. A comparative analysis using a PicoGreen® fluorescent quantification assay shows a comparable specificity for dsDNA quantification. Since the read-out of 96 samples with the DropSense96 is performed in 5 minutes without any prior mixing or incubation, this technology is a viable alternative to fluorescent quantification assays.

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Dye-free quantification of Oragene®/saliva DNA extracts using the Trinean DropSense96® Droplet Reader and cDrop™ Spectral Analysis Software