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Viruses and Viral Vaccines: Characterisation by Nanoparticle Tracking Analysis

Published 28 October 2014 | By Malvern Panalytical

NTA utilises the properties of both light scattering and Brownian motion in order to obtain the particle size distribution of samples in liquid suspension. A laser beam is passed through the sample chamber, and the particles in suspension in the path of this beam scatter light in such a manner that they can easily be visualised via a 20x magnification microscope onto which is mounted a camera. The camera, which operates at approximately 30 frames per second (fps), captures a video file of the particles moving under Brownian motion within the field of view of approximately 100μm x 80 μm x 10 μm (Figure 1).

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Viruses and Viral Vaccines: Characterisation by Nanoparticle Tracking Analysis