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Towards a Unified Biophysical Characterization Platform: Combining Dynamic Light Scattering and Raman Spectroscopy to Determine Protein Structure and Stability

Published 03 November 2015 | By Malvern Instruments

The combination of dynamic light scattering (DLS) with Raman spectroscopy has the capability to characterize a wealth of chemical, structural, and physical parameters of therapeutic proteins. Raman spectroscopy simultaneously derives protein secondary structure markers (amide I and III) and tertiary structure markers (aromatic side chains, disulfide bond, hydrogen bonding, local hydrophobicity). These markers can be monitored under controlled conditions by the determination of spectral peak position, shape, and/or intensity. Raman is able to make these structural determinations at formulation concentrations, 50 mg/mL or greater, rather than at the diluted concentrations required by conventional methods, i.e. typically less than a few mg/mL for circular dichroism (CD).

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Towards a Unified Biophysical Characterization Platform: Combining Dynamic Light Scattering and Raman Spectroscopy to Determine Protein Structure and Stability