Antibody Arrays

Proteins are major actors involved in many physiological processes. Variations in mRNA expression do not necessarily reflect the protein abundance in cells. Therefore, your DNA array data require further confirmation by antibody arrays (1).

You have a unique opportunity to assess real variations of protein abundance (up- and down-expression) and phosphorylation in healthy versus diseased, or in treated versus non-treated, biological samples using antibody arrays (2). ProtNeteomix proposes commercialised mAb arrays (MAGYarray) and its service to fabricate customised antibody arrays.

MAGYarray

Monoclonal antibody microarray designed for protein profiling in cells, biopsies, and biological fluids. Targeting 128 proteins of cell cycle, cytoskeleton, and signalling pathways. Two-colour detection with visible or near-infrared fluorescence dyes.  Rigorous data analysis by request.

Customised antibody arrays

Proteins extracted from total lysates or sub-cellular fractions can be analyzed with antibody arrays prepared by immobilization of each antibody in multiplicate spots. Know-how established binding conditions to decrease cross-reactivity of antibodies.

Near-infrared detection (700 nm & 800 nm) is preferentially used in our Company as it provides lower noise effect (3, 4). Monoclonal antibody arrays (sandwich technique) have been also developed to assess protein phosphorylation.

References:

1. Sakanyan V. J. Chromatography B 815, 77-95 (2005).
2. Yeretssian G. et al.  Mol. Cell. Proteomics 4, 605-617 (2005).
3. Snapyan M. et al. Proteomics 3, 647-657 (2003).
4. Sakanyan V. and Yeretssian G. In: Spectral Techniques in Proteomics. Ed. D. Sem, CRC Press, p. 185-205 (2007).

For more details see our website or contact us.