This is a novel CE application for ultrasensitive
Microfluidic Capillary Electrophoresis analysis of
multiple DNA fragments using integrated optics
and modulated laser beams in combination with
Fourier Transform analysis.

An innovative microfluidic chip with monolithically integrated waveguides for controlled excitation and off-chip fluorescence detection was applied on the commercially available Capella MCE platform of CapiliX BV.

The laser induced fluorescence (LIF) detection is combined with a unique single-point, multiwavelength modulated frequency encoding detection technique, enhanced by Fourier Transform analysis. Multiple fluorescently end labeled DNA fragments
of different lengths were obtained by selective PCR amplification of specific regions of genes using Multiplex Ligation-dependent Probe Amplification (MLPA). Diagnostically relevant genes related to breast cancer and other important diseases have been investigated.