Accurate total protein quantitation of purified proteins, cell lysates, affinity-column fractions or other complex protein samples is crucial for robust biochemical testing during biopharmaceutical development and production.

As the presence of chemicals does not allow simple A280 protein quantification, more labour-intensive alternatives for quantification are used.

Examples include the colourimetric Bradford or BCA assay, which negates to a large extent the presence of contaminants but requires multiple steps and shows variation depending on the sample composition and time point of measuring within the ongoing reaction.