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HPLC Analysis of Fluorescently labelled N-Glycans

Published 13 July 2011 | By Ludger

To enable the detection following separation of N-glycans by HPLC, the glycans are flourescently labelled with aryl amine compounds by reductive amination. The most commonly used flourescent label is 2-aminobenzamide (2AB), although 2-aminobenzoic acid (2-AA) and 2-aminopyride (2AP) are also used. The labelled glycans can then be analysed by a range of HPLC methods.

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HPLC Analysis of Fluorescently labelled N-Glycans