International Flavors & Fragrances Inc (IFF) has developed a state of the art fractionation process using the most modern equipment for this purpose. Key components of this system are the Sepbox® instrument made by Sepiatec GmbH (Germany) and the Genevac® HT-24 (Genevac Ltd., UK). The Sepbox allows for two dimensional, preparative scale fractionation of natural products using high performance liquid chromatography – solid phase extraction (HPLC-SPE). In sequential HPLC-SPE / HPLC-SPE steps individual components are isolated from complex mixtures. The Sepbox automates all of these stages.