The study finds selective pharmacological inhibition of specific arms of the ß2AR signaling network to correlate the differential contribution of signaling events to specific components of the cell response.
The essential role of intracellular Ca2+ in the cell response also led to the discovery of a novel ß2AR-promoted Ca2+ mobilization event, the researchers reported.
The study emphasizes the power of using cell monitoring to dissect the pluridimensionality of GPCR signaling using integrative approaches for a comprehensive readout of drug-induced cellular activity.