Log in or Register for enhanced features | Forgotten Password?
White Papers | Suppliers | Events | Report Store | Companies | Dining Club | Medical Devices | Videos
Pharmaceutical Business Review
Return to: PBR Home
Latest White Papers
Improved library preparation with the NEBNext® Ultra II DNA Library Prep Kit for Illumina™®
| By New England Biolabs
Here we demonstrate the utility of the NEBNext Ultra II DNA Library Prep Kit for DNA library construction from a variety of sample types and for a number of applications.
White Papers
1.5 Specific dsDNA Quantification UV/VIS-based cDrop™ Method vs Picogreen® Fluorescent Assay
| By Trinean
Precise quantification and normalization of isolated genomic DNA is critical for advanced molecular testing in life science research and molecular diagnostics. UV-Vis based quantification has long been the method of choice, mainly because of its convenience. However, determining the DNA concentration based on the absorbance at 260nm suffers from interference of components such as RNA, proteins and phenols. Alternative methods, such as the PicoGreen® based Quant-iT™ assay, based on fluorescence enhancement upon binding with dsDNA have been developed to address this issue.
Drug Research > Drug Discovery & Development > White Papers
1.3 Micro-volume Protein Quantification using the DropSense96 Droplet Plate Reader
| By Trinean
The measurement of protein concentration in aqueous samples is an important assay in biochemistry research and protein production facilities. Spectrophotometric protein quantification assays are commonly used methods to rapidly determine the concentration of a protein. They utilize the direct UV absorbance of the protein at 280nm in combination with its extinction coefficient or an indirect dye-based methods like BCA, Lowry and Bradford assays.
Drug Research > Drug Discovery & Development > White Papers
1.2 DropSense96 and its DropPlates, a Versatile Tool for Nucleic Acid and Protein Droplet Quantification
| By Trinean
As the demand for molecular tests increases, automated systems for biomolecule isolation and sample analysis are becoming popular. These facilitate integration of the complete molecular test in a full automatic set-up with liquid robots and bar-coded sample tracking resulting in a significant higher throughput while minimizing tedious manual repetitive tasks. The Trinean technology is a unique combination of the DropSense96 droplet plate reader, the 96well DropPlate microfuidic consumables and the DropQuant analytical software enabling direct quantification of small biomolecule samples in these high throughput, automated workflow.
Drug Research > Drug Discovery & Development > White Papers
1.14 Droplet UV/VIS Quantification of Native Proteins in Biopharmaceutical Processing
| By Trinean
Biopharmaceuticals are mainly produced by microbial fermentation or in animal cell cultures. As a consequence, a cascade of procedures including purification and characterization are required throughout the processing chain to obtain the protein at the desired level of purity and potency. Within these processes, quantification of the protein of interest is mandatory at critical steps to maintain confidence in the processing results and fidelity in the end product.
Drug Research > Drug Discovery & Development > White Papers
1.20 Quantification of His-tagged IgG antibodies after IMAC Ni-column purification
| By Trinean
Trinean introduces a new cDrop™ application suited for the quantification (OD280) of IgG antibodies and derivatives like Fab fragments during purification by IMAC Ni-columns using imidazole-containing buffers.
Drug Research > Drug Discovery & Development > White Papers
Dye-free quantification of Oragene®/saliva DNA extracts using the Trinean DropSense96® Droplet Reader and cDrop™ Spectral Analysis Software
| By Trinean
Saliva samples collected with Oragene® self-collection kits from DNA Genotek offer a non-invasive and proven method for accessing high quality and quantity DNA for genomic testing and analysis. Proper quantification of the extracted DNA is a critical step for most downstream applications. Trinean has developed a specific quantification protocol for DNA from Oragene/saliva samples using the DropSense96® droplet reader and the cDrop™ spectral decomposition software. A comparative analysis using a PicoGreen® fluorescent quantification assay shows a comparable specificity for dsDNA quantification. Since the read-out of 96 samples with the DropSense96 is performed in 5 minutes without any prior mixing or incubation, this technology is a viable alternative to fluorescent quantification assays.
Drug Research > Drug Discovery & Development > White Papers
1.17 Maxwell®16 Blood purification Kit App and Maxwell®16 LEV Blood DNA Kit App
| By Trinean
A spectral content profiling application on the cDrop™ software is available for quantification and contamination analysis of DNA isolations from blood using the Maxwell® 16 Blood DNA purification Kit and The Maxwell®16 LEV Blood DNA Kit. In this note we describe how to use this application and how to export and interpret the results.
Drug Research > Drug Discovery & Development > White Papers
1.7 Quantifying Amplicons in Non-Purified PCR Reactions: Direct Assessment of Product Yield
| By Trinean
The polymerase chain reaction (PCR) is a fundamental tool in molecular biology and clinical testing. Most genetic tests rely upon the power of PCR to enrich specific target-sequences prior to analysis. Analytical methods like sequencing are relatively expensive and labs want to minimize the resources spent on samples that don't meet the requirements of concentration and quality to deliver a reliable result.
Drug Research > Drug Discovery & Development > White Papers
1.19 Qiagen® EZ1 Tissue DNA app
| By Trinean
A spectral content profiling application on the cDrop™ software is available for quantification and contamination analysis of DNA isolations from cells using the Qiagen® EZ1 Tissue DNA kit. In this note we describe how to use this application and how to export and interpret the results.
Drug Research > Drug Discovery & Development > White Papers
See all White Papers