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Protein Storage and Stability Analysis Using the Zetasizer Nano ZSP
| By Malvern Instruments
Spidroins are a unique family of large, structural proteins that make up the bulk of spider silk fibres. The mechanical strength and elasticity of spider silk fibres have led to their successful use in the regeneration of peripheral nerves in rats [1], with the same properties giving the potential for use in a wide range of other applications [2,3,4]. The production of these proteins on an industrial scale, however, has only become possible fairly recently.
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Using Differential Scanning Calorimetry to Inform and Accelerate Process Development for Protein Therapeutics
| By Malvern Instruments
The production and purification of immunoglobulins of the gamma isotype, particularly human IgG1, for diagnostic or therapeutic applications is now fairly routine. In the past decade IgE-based therapeutics have also gained momentum (1-3). IgE is important for host defense against parasites and for protective inflammation. Yet, IgE-mediated signaling through its receptors is also a focal point of inflammatory allergic disease (4). The constant region of IgE, is a homodimer containing duplicate pairs of three unique Ig-fold domains (Cε2, Cε3, and Cε4), and is responsible for binding its two receptors, FcεRI and CD23, also known as FcεRII.
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Using Differential Scanning Calorimetry to Inform and Accelerate Process Development for Protein Therapeutics
| By Malvern Instruments
The production and purification of immunoglobulins of the gamma isotype, particularly human IgG1, for diagnostic or therapeutic applications is now fairly routine. In the past decade IgE-based therapeutics have also gained momentum (1-3). IgE is important for host defense against parasites and for protective inflammation. Yet, IgE-mediated signaling through its receptors is also a focal point of inflammatory allergic disease (4). The constant region of IgE, is a homodimer containing duplicate pairs of three unique Ig-fold domains (Cε2, Cε3, and Cε4), and is responsible for binding its two receptors, FcεRI and CD23, also known as FcεRII.
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Evaluating Product Texture Using Oscillatory Testing on a Rotational Rheometer
| By Malvern Instruments
Formulating products to have both the correct functional and sensory attributes can be a difficult task. The latter in particular relies heavily on user feedback which can take considerable time and effort to attain. In addition it is not always easy to interpret such feedback in the context of material properties and hence rheological data.
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Improved library preparation with the NEBNext® Ultra II DNA Library Prep Kit for Illumina™®
| By New England Biolabs
Here we demonstrate the utility of the NEBNext Ultra II DNA Library Prep Kit for DNA library construction from a variety of sample types and for a number of applications.
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1.5 Specific dsDNA Quantification UV/VIS-based cDrop™ Method vs Picogreen® Fluorescent Assay
| By Trinean
Precise quantification and normalization of isolated genomic DNA is critical for advanced molecular testing in life science research and molecular diagnostics. UV-Vis based quantification has long been the method of choice, mainly because of its convenience. However, determining the DNA concentration based on the absorbance at 260nm suffers from interference of components such as RNA, proteins and phenols. Alternative methods, such as the PicoGreen® based Quant-iT™ assay, based on fluorescence enhancement upon binding with dsDNA have been developed to address this issue.
Drug Research > Drug Discovery & Development > White Papers
1.3 Micro-volume Protein Quantification using the DropSense96 Droplet Plate Reader
| By Trinean
The measurement of protein concentration in aqueous samples is an important assay in biochemistry research and protein production facilities. Spectrophotometric protein quantification assays are commonly used methods to rapidly determine the concentration of a protein. They utilize the direct UV absorbance of the protein at 280nm in combination with its extinction coefficient or an indirect dye-based methods like BCA, Lowry and Bradford assays.
Drug Research > Drug Discovery & Development > White Papers
1.2 DropSense96 and its DropPlates, a Versatile Tool for Nucleic Acid and Protein Droplet Quantification
| By Trinean
As the demand for molecular tests increases, automated systems for biomolecule isolation and sample analysis are becoming popular. These facilitate integration of the complete molecular test in a full automatic set-up with liquid robots and bar-coded sample tracking resulting in a significant higher throughput while minimizing tedious manual repetitive tasks. The Trinean technology is a unique combination of the DropSense96 droplet plate reader, the 96well DropPlate microfuidic consumables and the DropQuant analytical software enabling direct quantification of small biomolecule samples in these high throughput, automated workflow.
Drug Research > Drug Discovery & Development > White Papers
1.14 Droplet UV/VIS Quantification of Native Proteins in Biopharmaceutical Processing
| By Trinean
Biopharmaceuticals are mainly produced by microbial fermentation or in animal cell cultures. As a consequence, a cascade of procedures including purification and characterization are required throughout the processing chain to obtain the protein at the desired level of purity and potency. Within these processes, quantification of the protein of interest is mandatory at critical steps to maintain confidence in the processing results and fidelity in the end product.
Drug Research > Drug Discovery & Development > White Papers
1.20 Quantification of His-tagged IgG antibodies after IMAC Ni-column purification
| By Trinean
Trinean introduces a new cDrop™ application suited for the quantification (OD280) of IgG antibodies and derivatives like Fab fragments during purification by IMAC Ni-columns using imidazole-containing buffers.
Drug Research > Drug Discovery & Development > White Papers
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