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New England Biolabs, Inc. - Advanced Resources for Drug Discovery & Development White Papers

New England Biolabs, Inc. - Advanced Resources for Drug Discovery & Development

Improved library preparation with the NEBNext® Ultra II DNA Library Prep Kit for Illumina™® Here we demonstrate the utility of the NEBNext Ultra II DNA Library Prep Kit for DNA library construction from a variety of sample types and for a number of applications.
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Minding your Caps and Tails – Considerations for Functional mRNA Synthesis Applications of synthetic mRNA have grown and become considerably diversified in recent years. Examples include the generation of pluripotent stem cells (1-3), vaccines and therapeutics (4), and CRISPR/Cas9 genome editing applications (5-7). The basic requirements for a functional mRNA - a 7-methylguanylate cap at the 5´ end and a poly(A) tail at the 3´ end - must be added in order to obtain efficient translation by eukaryotic cells. Additional considerations can include the incorporation of modified bases, modified cap structures and polyadenylation strategies. Strategies for in vitro synthesis of mRNA may also vary according to the desired scale of synthesis. This article discusses options for selection of reagents and the extent to which it influences synthesized mRNA functionality.
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